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  • Featured Product
  • KD/KO Validated

EEF1B2 Monoclonal antibody

EEF1B2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E1C11

Cat no : 60329-1-Ig

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

Validation Data Gallery

Applications:
  • WB analysis using 60329-1-Ig

    WB analysis using 60329-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HeLa using 60329-1-Ig

    WB analysis of HeLa using 60329-1-Ig

    WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

  • WB analysis of HeLa using 60329-1-Ig

    WB analysis of HeLa using 60329-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 60329-1-Ig

    WB analysis of HEK-293 using 60329-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 60329-1-Ig

    IF Staining of HeLa using 60329-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60329-1-Ig(EEF1B2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60329-1-Ig targets EEF1B2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0135 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight 25 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000211
Gene symbol EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2881438
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for EEF1B2 antibody 60329-1-IgDownload protocol
IF protocol for EEF1B2 antibody 60329-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols