EEA1 Recombinant antibody
EEA1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2C11
Cat no : 81245-1-RR
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
81245-1-RR targets EEA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種 |
| Full Name | early endosome antigen 1 |
| Calculated molecular weight | 162 kDa |
| Observed molecular weight | 162 kDa |
| GenBank accession number | NM_003566 |
| Gene symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EEA1 antibody 81245-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
