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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOCK7 Monoclonal antibody

DOCK7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A4A6

Cat no : 67842-1-Ig

Synonyms

dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2

Validation Data Gallery

Applications:
  • WB analysis using 67842-1-Ig

    WB analysis using 67842-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67842-1-Ig (DOCK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67842-1-Ig

    WB analysis using 67842-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67842-1-Ig (DOCK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, PC-12 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, NIH3T3 cells, 4T1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67842-1-Ig targets DOCK7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DOCK7 fusion protein Ag28417 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 7
Calculated molecular weight 2109 aa, 239 kDa
Observed molecular weight243 kDa
GenBank accession numberBC016392
Gene symbol DOCK7
Gene ID (NCBI) 85440
RRIDAB_2918604
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for DOCK7 antibody 67842-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols