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KD/KO Validated

DNAJB12 Polyclonal antibody

Name: DNAJB12 Polyclonal antibody
Brand: Proteintech
SKU: 16780-1-AP

DNAJB12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16780-1-AP

Synonyms

DJ10, DNAJB12

Validation Data Gallery

Applications:

AllWBIP

WB analysis using 16780-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 16780-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 16780-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16780-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16780-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16780-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 16780-1-AP
IP result of anti-DNAJB12 (IP:16780-1-AP, 4ug; Detection:16780-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Cat No. 16780-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, A549 cells, human brain tissue, human kidney tissue, human liver tissue, MCF-7 cells, MKN-45 cells, rat brain tissue
Positive IP detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IF	See 3 publications below
IP	See 3 publications below
ChIP	See 1 publications below

Product Information

16780-1-AP targets DNAJB12 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNAJB12 fusion protein Ag10193 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 12
Calculated molecular weight	375 aa, 42 kDa
Observed molecular weight	40-42 kDa
GenBank accession number	BC011812
Gene symbol	DNAJB12
Gene ID (NCBI)	54788
RRID	AB_2094404
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB12 antibody 16780-1-AP	Download protocol
IP protocol for DNAJB12 antibody 16780-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,ChIP	Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. Authors - Jin Zhou View Article
human	IP	Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. Authors - Erna Davydova View Article
	WB, IP	J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. Authors - Yu-Jie Chen View Article
human	WB	PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. Authors - Christopher Paul Walczak View Article
human	WB,IF	J Cell Biol Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication Authors - Jeffrey M Williams View Article
human	IF	J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. Authors - Parikshit Bagchi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB12 Polyclonal antibody

DNAJB12 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 16780-1-AP

WB analysis using 16780-1-AP

WB analysis using 16780-1-AP

WB analysis of human brain using 16780-1-AP

WB analysis of human kidney using 16780-1-AP

WB analysis of human liver using 16780-1-AP

IP experiment of mouse brain using 16780-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.

The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.

Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication

The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.