Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13174-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 13174-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 13174-1-AP
IP result of anti-DNAJB1 (IP:13174-1-AP, 4ug; Detection:13174-1-AP 1:500) with mouse lung tissue lysate 4000ug.
IHC staining of mouse testis using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13174-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13174-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (13174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 13174-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (13174-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 13174-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Heat shock 40 kDa protein 1, hDj-1, Hdj1, hDj 1, DNAJ1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse lung tissue, rat lung tissue, rat liver tissue, C2C12 cells, C6 cells
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human oesophagus cancer tissue, human lymphoma tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 32 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

13174-1-AP targets DNAJB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3830 Product name: Recombinant human DNAJB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC019827 Sequence: MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight	340 aa, 40 kDa
Observed molecular weight	37-40 kDa
GenBank accession number	BC019827
Gene Symbol	DNAJB1
Gene ID (NCBI)	3337
RRID	AB_2230742
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P25685
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
IF protocol for DNAJB1 antibody 13174-1-AP	Download protocol
IHC protocol for DNAJB1 antibody 13174-1-AP	Download protocol
IP protocol for DNAJB1 antibody 13174-1-AP	Download protocol
WB protocol for DNAJB1 antibody 13174-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality control Authors - Yingwei Ge View Article
rat	IF	Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination Authors - Li Li View Article
human	WB	Proc Natl Acad Sci U S A Local translation in nuclear condensate amyloid bodies. Authors - Phaedra R Theodoridis View Article
mouse	WB	Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction Authors - Jie Pan View Article
human	WB	Int J Mol Sci PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway. Authors - Yanqing Li View Article
	WB	Front Oncol A Prognostic Prediction Model Developed Based on Four CpG Sites and Weighted Correlation Network Analysis Identified DNAJB1 as a Novel Biomarker for Pancreatic Cancer. Authors - Lingming Kong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13174-1-AP

WB analysis using 13174-1-AP

IP experiment of mouse lung using 13174-1-AP

IHC staining of mouse testis using 13174-1-AP

IHC staining of human oesophagus cancer using 13174-1-AP

IHC staining of mouse brain using 13174-1-AP

IHC staining of human oesophagus cancer using 13174-1-AP

IHC staining of mouse testis using 13174-1-AP

IHC staining of human lymphoma using 13174-1-AP

IHC staining of human lymphoma using 13174-1-AP

IF Staining of HeLa using 13174-1-AP

FC experiment of HeLa using 13174-1-AP

DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Local translation in nuclear condensate amyloid bodies.

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

A Prognostic Prediction Model Developed Based on Four CpG Sites and Weighted Correlation Network Analysis Identified DNAJB1 as a Novel Biomarker for Pancreatic Cancer.