Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 17721-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17721-1-AP
IP result of anti-DHX9 (IP:17721-1-AP, 4ug; Detection:17721-1-AP 1:30000) with HeLa cells lysate 880 ug.
IHC staining of mouse testis using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 17721-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DHX9 Polyclonal antibody (17721-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

DHX9 Polyclonal antibody

DHX9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17721-1-AP

Publications(50)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Synonyms

ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13, LKP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (50)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, rat spleen tissue, rat testis tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 43 publications below
IHC	See 5 publications below
IF	See 9 publications below
IP	See 4 publications below
ELISA	See 1 publications below
CoIP	See 4 publications below
ChIP	See 1 publications below
RIP	See 5 publications below

Product Information

17721-1-AP targets DHX9 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12104 Product name: Recombinant human DHX9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC014246 Sequence: MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPP 相同性解析による交差性が予測される生物種
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight	1270 aa, 141 kDa
Observed molecular weight	140 kDa
GenBank accession number	BC014246
Gene Symbol	DHX9
Gene ID (NCBI)	1660
RRID	AB_2092506
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08211
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
FC protocol for DHX9 antibody 17721-1-AP	Download protocol
IF protocol for DHX9 antibody 17721-1-AP	Download protocol
IHC protocol for DHX9 antibody 17721-1-AP	Download protocol
IP protocol for DHX9 antibody 17721-1-AP	Download protocol
WB protocol for DHX9 antibody 17721-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Hepatology Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma. Authors - Zhixiang Hu View Article
mouse	WB	Sci Adv R-loops coordinate with SOX2 in regulating reprogramming to pluripotency. Authors - Yaoyi Li View Article
human	RIP	Nat Commun Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1. Authors - Hamed Kooshapur View Article
human	WB	Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Authors - Roy Matkovic View Article
human	WB	Nat Commun Interaction of 7SK with the Smn complex modulates snRNP production. Authors - Changhe Ji View Article
human	WB	Nat Commun PMID: 31792210 Authors - Sunwang Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17721-1-AP

WB analysis using 17721-1-AP

WB analysis using 17721-1-AP

WB analysis of rat spleen using 17721-1-AP

IP experiment of HeLa using 17721-1-AP

IHC staining of mouse testis using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse testis using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

FC experiment of HepG2 using 17721-1-AP

DHX9 Polyclonal antibody

DHX9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.

Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Interaction of 7SK with the Smn complex modulates snRNP production.

PMID: 31792210

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