DHCR24 Monoclonal antibody
DHCR24 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2F6E11
Cat no : 60028-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
60028-1-Ig targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DHCR24 fusion protein Ag0756 相同性解析による交差性が予測される生物種 |
| Full Name | 24-dehydrocholesterol reductase |
| Calculated molecular weight | 60 kDa, 516 aa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC004375 |
| Gene symbol | DHCR24 |
| Gene ID (NCBI) | 1718 |
| RRID | AB_874115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DHCR24 antibody 60028-1-Ig | Download protocol |
| IHC protocol for DHCR24 antibody 60028-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
