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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D3D2

Cat no : CL594-67025

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67025

    IF Staining of HepG2 using CL594-67025

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated DDX5 antibody (CL594-67025, Clone: 2D3D2 ) at dilution of 1:100.

  • FC experiment of HeLa using CL594-67025

    FC experiment of HeLa using CL594-67025

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human DDX5 (CL594-67025, Clone:2D3D2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67025-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67025 targets DDX5 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX5 fusion protein Ag24821 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Observed molecular weight 69 kDa
GenBank accession numberNM_004396
Gene symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2920073
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 DDX5 antibody CL594-67025Download protocol
FC protocol for CL594 DDX5 antibody CL594-67025Download protocol
Standard Protocols
Click here to view our Standard Protocols