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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX3 Recombinant antibody

DDX3 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C12

Cat no : 81903-1-RR

Synonyms

DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2

Validation Data Gallery

Applications:
  • WB analysis using 81903-1-RR

    WB analysis using 81903-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 81903-1-RR

    IF Staining of HepG2 using 81903-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

81903-1-RR targets DDX3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX3 fusion protein Ag1614 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated molecular weight 73 kDa
Observed molecular weight75 kDa
GenBank accession numberBC011819
Gene symbol DDX3X
Gene ID (NCBI) 1654
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Protocols

Product Specific Protocols
WB protocol for DDX3 antibody 81903-1-RRDownload protocol
IF protocol for DDX3 antibody 81903-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols