CoraLite® Plus 488-conjugated Cytoglobin Monoclonal antibody
Cytoglobin Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4C7E11
Cat no : CL488-60228
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 60228-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-60228 targets Cytoglobin in IF applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Reactivity | human, mouse, pig, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytoglobin fusion protein Ag4161 相同性解析による交差性が予測される生物種 |
| Full Name | cytoglobin |
| Calculated molecular weight | 190 aa, 21 kDa |
| Observed molecular weight | 27-29 kDa |
| GenBank accession number | BC029798 |
| Gene symbol | CYGB |
| Gene ID (NCBI) | 114757 |
| RRID | AB_2883122 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Cytoglobin antibody CL488-60228 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
