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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclin E1 Polyclonal antibody

Cyclin E1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11554

Synonyms

CCNE, CCNE1, cyclin E1, G1/S specific cyclin E1

Validation Data Gallery

Applications:
  • FC experiment of MCF-7 using CL488-11554

    FC experiment of MCF-7 using CL488-11554

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cyclin E1 (CL488-11554) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-11554

    FC experiment of HeLa using CL488-11554

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cyclin E1 (CL488-11554) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inMCF-7 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11554-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-11554 targets Cyclin E1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin E1 fusion protein Ag2110 相同性解析による交差性が予測される生物種
Full Name cyclin E1
Calculated molecular weight 410 aa, 47 kDa
GenBank accession numberBC035498
Gene symbol CCNE1
Gene ID (NCBI) 898
RRIDAB_2919033
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Cyclin E1 antibody CL488-11554Download protocol
Standard Protocols
Click here to view our Standard Protocols