CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody
CYP2E1-Specific Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
mouse, rat, pig, human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F12G7
Cat no : CL488-67263
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | mouse liver tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67263 targets CYP2E1-Specific in IF, FC (Intra) applications and shows reactivity with mouse, rat, pig, human samples.
| Tested Reactivity | mouse, rat, pig, human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 50-57 kDa |
| GenBank accession number | NM_000773 |
| Gene symbol | CYP2E1 |
| Gene ID (NCBI) | 1571 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263 | Download protocol |
| FC protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
