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CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig, human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F12G7

Cat no : CL488-67263

Synonyms

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated CYP2E1-Specific Mono (CL488-67263)

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated CYP2E1-Specific Mono (CL488-67263)

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CYP2E1-Specific Mono (CL488-67263)

    FC experiment of HeLa using CL488-67263

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CYP2E1-Specific (CL488-67263, Clone:1F12G7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse liver tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67263 targets CYP2E1-Specific in IF, FC (Intra) applications and shows reactivity with mouse, rat, pig, human samples.

Tested Reactivity mouse, rat, pig, human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberNM_000773
Gene Symbol CYP2E1
Gene ID (NCBI) 1571
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263Download protocol
FC protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263Download protocol
Standard Protocols
Click here to view our Standard Protocols