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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12916

Synonyms

CUL 4B, CUL4B, cullin 4B, DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL594-12916

    FC experiment of HeLa using CL594-12916

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CUL4B (CL594-12916) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12916-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-12916 targets CUL4B in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL4B fusion protein Ag3563 相同性解析による交差性が予測される生物種
Full Name cullin 4B
Calculated molecular weight 913 aa, 104 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC036216
Gene symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_2919790
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Protocols

Product Specific Protocols
FC protocol for CL594 CUL4B antibody CL594-12916Download protocol
Standard Protocols
Click here to view our Standard Protocols