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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREB3L4 Polyclonal antibody

CREB3L4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13630-1-AP

Synonyms

AIBZIP, ATCE1, Attaching to CRE like 1, CREB 4, CREB3, CREB3L4, CREB4, hJAL, JAL, Tisp40

Validation Data Gallery

Applications:
  • WB analysis using 13630-1-AP

    WB analysis using 13630-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 13630-1-AP

    WB analysis of HL-60 using 13630-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13630-1-AP

    IF Staining of MCF-7 using 13630-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13630-1-AP (CREB3L4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L4 Ready-To-Use IHC Kit. CREB3L4 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13630-1-AP targets CREB3L4 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB3L4 fusion protein Ag4548 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 4
Calculated molecular weight 395 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC038962
Gene symbol CREB3L4
Gene ID (NCBI) 148327
RRIDAB_2276550
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREB3L4 (cAMP responsive element binding protein 3-like 4), also named as AIBZIP, CREB4 and ATCE1, is a member of the CREB/ATF transcription factor family. CREB3L4 may play a role in the unfolded protein response, characterized by their regulation of gene expression through the cAMP-responsive element(PMID: 22829733). CREB3L4 is highly expressed in multiple human cancers, such as human breast carcinoma, prostate cancer(PMID: 32026099, PMID: 28338058). The molecular weight of CREB3L4 is 43 kDa and 55 kDa precursor was reported. Due to glycosylation, CREB3L4 can be observed with molecular weights of 35 and 41 kDa (PMID: 25412305, 15938716).

Protocols

Product Specific Protocols
WB protocol for CREB3L4 antibody 13630-1-APDownload protocol
IF protocol for CREB3L4 antibody 13630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols