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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COQ6 Monoclonal antibody

COQ6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5B1

Cat no : 67162-1-Ig

Synonyms

CGI 10, COQ6

Validation Data Gallery

Applications:
  • WB analysis of human heart using 67162-1-Ig

    WB analysis of human heart using 67162-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 67162-1-Ig

    WB analysis of pig brain using 67162-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 67162-1-Ig

    WB analysis of mouse brain using 67162-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 67162-1-Ig

    WB analysis of rat heart using 67162-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig heart using 67162-1-Ig

    WB analysis of pig heart using 67162-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 67162-1-Ig

    IHC staining of rat heart using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat heart using 67162-1-Ig

    IHC staining of rat heart using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67162-1-Ig

    IHC staining of human liver cancer using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67162-1-Ig

    IHC staining of human liver cancer using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman heart tissue, mouse brain tissue, pig brain tissue, pig heart tissue, rat heart tissue
Positive IHC detected inrat heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67162-1-Ig targets COQ6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COQ6 fusion protein Ag28991 相同性解析による交差性が予測される生物種
Full Name coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
Calculated molecular weight 468 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC014181
Gene symbol COQ6
Gene ID (NCBI) 51004
RRIDAB_2882458
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COQ6 antibody 67162-1-IgDownload protocol
IHC protocol for COQ6 antibody 67162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols