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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CNPY2, MSAP Monoclonal antibody

CNPY2, MSAP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G8G11

Cat no : CL488-66173

Synonyms

canopy 2 homolog (zebrafish), CNPY2, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-66173

    IF Staining of HepG2 using CL488-66173

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66173 (CNPY2, MSAP antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66173-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66173 targets CNPY2, MSAP in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNPY2, MSAP fusion protein Ag6363 相同性解析による交差性が予測される生物種
Full Name canopy 2 homolog (zebrafish)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC065015
Gene symbol CNPY2
Gene ID (NCBI) 10330
RRIDAB_2883276
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CNPY2, MSAP antibody CL488-66173Download protocol
Standard Protocols
Click here to view our Standard Protocols