• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H5H3

Cat no : CL488-67798

Synonyms

CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H

Validation Data Gallery

Applications:
  • IF Staining of human colon cancer using CL488-67798

    IF Staining of human colon cancer using CL488-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67798-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67798 targets CNOT4 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNOT4 fusion protein Ag29964 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 4
Calculated molecular weight 575 aa, 64 kDa
GenBank accession numberBC035590
Gene symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2919550
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CNOT4 antibody CL488-67798Download protocol
Standard Protocols
Click here to view our Standard Protocols