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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHMP7 Monoclonal antibody

CHMP7 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8F6

Cat no : 68406-1-Ig

Synonyms

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

Validation Data Gallery

Applications:
  • WB analysis using 68406-1-Ig

    WB analysis using 68406-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68406-1-Ig (CHMP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68406-1-Ig

    IF Staining of A431 using 68406-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (68406-1-Ig, Clone: 2F8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68406-1-Ig targets CHMP7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHMP7 fusion protein Ag9641 相同性解析による交差性が予測される生物種
Full Name CHMP family, member 7
Calculated molecular weight 453 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC019110
Gene symbol CHMP7
Gene ID (NCBI) 91782
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP7 antibody 68406-1-IgDownload protocol
IHC protocol for CHMP7 antibody 68406-1-IgDownload protocol
IF protocol for CHMP7 antibody 68406-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols