Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 16424-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 16424-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spinal cord using 16424-1-AP
mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat spinal cord using 16424-1-AP
rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 16424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 16424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 16424-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16424-1-AP (CHMP7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 16424-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A431 using 16424-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16424-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells
Positive IHC detected in	human kidney tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 5 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

16424-1-AP targets CHMP7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9625 Product name: Recombinant human CHMP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-453 aa of BC019110 Sequence: ELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAELEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL 相同性解析による交差性が予測される生物種
Full Name	CHMP family, member 7
Calculated molecular weight	453 aa, 51 kDa
Observed molecular weight	51-55 kDa
GenBank accession number	BC019110
Gene Symbol	CHMP7
Gene ID (NCBI)	91782
RRID	AB_2079500
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WUX9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CHMP7 antibody 16424-1-AP	Download protocol
IHC protocol for CHMP7 antibody 16424-1-AP	Download protocol
WB protocol for CHMP7 antibody 16424-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival Authors - Pauline Lascaux View Article
human	WB,IF	Nat Cell Biol Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation. Authors - Marina Vietri KD Validated View Article
human	WB	Dev Cell CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. Authors - Leandro N Ventimiglia KD Validated View Article
human	WB	Dev Cell The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Authors - Samuel S Wallis KD Validated View Article
human	WB	Curr Biol Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation. Authors - Yolanda Olmos KD Validated View Article
human	WB,IP	Elife CDK1 controls CHMP7-dependent nuclear envelope reformation. Authors - Alberto T Gatta KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16424-1-AP

WB analysis using 16424-1-AP

WB analysis of mouse spinal cord using 16424-1-AP

WB analysis of rat spinal cord using 16424-1-AP

IHC staining of human kidney using 16424-1-AP

IHC staining of human kidney using 16424-1-AP

IHC staining of human colon cancer using 16424-1-AP

IF Staining of A431 using 16424-1-AP

IF Staining of A431 using 16424-1-AP

CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.

CDK1 controls CHMP7-dependent nuclear envelope reformation.