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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H3A4

Cat no : CL488-67145

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-67145

    FC experiment of Jurkat using CL488-67145

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC25B (CL488-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67145-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67145 targets CDC25B in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC25B fusion protein Ag27193 相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated molecular weight 65 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC006395
Gene symbol CDC25B
Gene ID (NCBI) 994
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CDC25B antibody CL488-67145Download protocol
Standard Protocols
Click here to view our Standard Protocols