• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat no : 67145-1-Ig

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

Validation Data Gallery

Applications:
  • WB analysis of Sp2/0 using 67145-1-Ig

    WB analysis of Sp2/0 using 67145-1-Ig

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67145-1-Ig

    WB analysis of Jurkat using 67145-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 67145-1-Ig

    WB analysis of U-937 using 67145-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 67145-1-Ig

    IF Staining of Jurkat using 67145-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inSp2/0 cells, Jurkat cells, U-937 cells
Positive IF detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67145-1-Ig targets CDC25B in WB, IF, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC25B fusion protein Ag27193 相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated molecular weight 65 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC006395
Gene symbol CDC25B
Gene ID (NCBI) 994
RRIDAB_2882444
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Protocols

Product Specific Protocols
WB protocol for CDC25B antibody 67145-1-IgDownload protocol
IF protocol for CDC25B antibody 67145-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols