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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

53-6.7

Cat no : CL647-65069

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

Validation Data Gallery

Applications:
  • IF Staining of mouse splenocytes using CL647-65069

    IF Staining of mouse splenocytes using CL647-65069

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD8a (CL647-65069, Clone: 53-6.7) at dilution of 1:500.

  • FC experiment of mouse splenocytes using CL647-65069

    FC experiment of mouse splenocytes using CL647-65069

    1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65069, Clone:53-6.7) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65069-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65069 targets CD8a in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Spleen cells or thymocyte membranes 相同性解析による交差性が予測される生物種
Full Name CD8 antigen, alpha chain
GenBank accession numberBC030679
Gene symbol Cd8a
Gene ID (NCBI) 12525
RRIDAB_2883664
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD8a antibody CL647-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols