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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD68 Polyclonal antibody

CD68 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25747

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL488-25747

    IF Staining of human tonsillitis using CL488-25747

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD68 antibody (CL488-25747) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-25747

    IF Staining of human tonsillitis using CL488-25747

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD68 antibody (CL488-25747) at dilution of 1:200.

  • FC experiment of RAW 264.7 using CL488-25747

    FC experiment of RAW 264.7 using CL488-25747

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Human CD68 (CL488-25747) (red), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25747-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-25747 is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
RRIDAB_2919205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD68 antibody CL488-25747Download protocol
Standard Protocols
Click here to view our Standard Protocols