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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD68 Polyclonal antibody

CD68 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 30929-1-AP

Synonyms

Cd68, CD68 antigen, gp110, Scard1

Validation Data Gallery

Applications:
  • IHC staining of mouse spleen using 30929-1-AP

    IHC staining of mouse spleen using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 30929-1-AP

    IHC staining of mouse spleen using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse liver using 30929-1-AP

    IHC staining of mouse liver using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse liver using 30929-1-AP

    IHC staining of mouse liver using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included

Tested Applications

Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

30929-1-AP targets CD68 in IHC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
GenBank accession numberNM_001291058.1
Gene symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
IHC protocol for CD68 antibody 30929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols