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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD5L Polyclonal antibody

CD5L Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17224

Synonyms

AIM, API6, CD5 antigen like, CD5 molecule like, CD5L, CT 2, IgM associated peptide, PRO229, SP ALPHA, Spalpha

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-17224

    IF Staining of HepG2 using CL488-17224

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CD5L antibody (CL488-17224) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 17224-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17224 targets CD5L in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD5L fusion protein Ag11062 相同性解析による交差性が予測される生物種
Full Name CD5 molecule-like
Calculated molecular weight 347 aa, 38 kDa
Observed molecular weight40 kDa
GenBank accession numberBC033586
Gene symbol CD5L
Gene ID (NCBI) 922
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD5L antibody CL488-17224Download protocol
Standard Protocols
Click here to view our Standard Protocols