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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Human, Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

RA3-6B2

Cat no : CL488-65139

Synonyms

B220, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

Validation Data Gallery

Applications:
  • IF Staining of mouse splenocytes using CL488-65139

    IF Staining of mouse splenocytes using CL488-65139

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated CD45R (B220' target='_blank'>B220) antibody (CL488-65139, Clone: RA3-6B2, green) at dilution of 1:400, and CoraLite® Plus 647-conjugated CD3 antibody (CL647-65077, Clone: 17A2, red).

  • FC experiment of mouse splenocytes using CL488-65139

    FC experiment of mouse splenocytes using CL488-65139

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated Rat IgG2a isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD45R (B220' target='_blank'>B220) (CL488-65139, Clone: RA3-6B2) (right). Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65139-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65139 targets CD45R (B220) in IF, FC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC028512
Gene symbol Ptprc
Gene ID (NCBI) 19264
RRIDAB_2883199
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD45R (B220) antibody CL488-65139Download protocol
FC protocol for CL Plus 488 CD45R (B220) antibody CL488-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols