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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI30

Cat no : APC-65109

Synonyms

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using APC-65109

    FC experiment of human PBMCs using APC-65109

    1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65109

    FC experiment of human PBMCs using APC-65109

    1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of Raji using APC-65109

    FC experiment of Raji using APC-65109

    1X10^6 Raji cells were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30) (red), or 5 ul Control Antibody. Cells were not fixed .

Tested Applications

Positive FC detected inhuman PBMCs, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

APC-65109 targets CD45 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human peripheral blood leucocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
RRIDAB_2882998
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for APC CD45 antibody APC-65109Download protocol
Standard Protocols
Click here to view our Standard Protocols