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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

GK1.5

Cat no : CL750-65104

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL750-65104

    FC experiment of mouse splenocytes using CL750-65104

    1X10^6 mouse splenocytes were surface co-stained with CoraLite®488 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 750 Anti-Mouse CD4 (CL750-65104, Clone: GK1.5) or 0.5 ug Isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65104-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL750-65104 targets CD4 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CTL clone V4 相同性解析による交差性が予測される生物種
Full Name CD4 antigen
GenBank accession numberBC039137
Gene symbol Cd4
Gene ID (NCBI) 12504
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD4 antibody CL750-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols