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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD357 (GITR) (DTA-1)

CD357 (GITR) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

DTA-1

Cat no : CL647-65102

Synonyms

AITR, CD357, CD357 (GITR), Gitr, Tnfrsf18

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL647-65102

    FC experiment of mouse splenocytes using CL647-65102

    1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite®488-Conjugated Anti-Mouse CD25 (CL488-65137, Clone: PC61.5) and 5 ul CoraLite® Plus 647 Anti-Mouse CD357 (GITR) (CL647-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65102-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65102 targets CD357 (GITR) in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CD25+ CD4+ T cells 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 18
GenBank accession numberBC146517
Gene symbol Tnfrsf18
Gene ID (NCBI) 21936
RRIDAB_2883680
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD357 (GITR) antibody CL647-65102Download protocol
Standard Protocols
Click here to view our Standard Protocols