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CoraLite® Plus 488 Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

PC61.5

Cat no : CL488-65137

Synonyms

CD25, Il2r, Il2ra, Il-2ra, Ly 43

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD25 (PC61.5) (CL488-65137)

    FC experiment of mouse splenocytes using CL488-65137

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.125 ug CoraLite® Plus 488 Anti-Mouse CD25 (CL488-65137, Clone: PC61.5) or 0.125 ug CoraLite® Plus 488-conjugated Rat IgG1 isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, lambda
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name interleukin 2 receptor, alpha chain
GenBank Accession NumberBC114437
Gene Symbol Il2ra
Gene ID (NCBI) 16184
RRIDAB_2883198
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD25 antibody CL488-65137Download protocol
Standard Protocols
Click here to view our Standard Protocols