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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD31 Polyclonal antibody

CD31 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11265

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL488-11265

    IF Staining of human tonsillitis using CL488-11265

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-11265

    IF Staining of human tonsillitis using CL488-11265

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11265-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11265 targets CD31 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
GenBank accession numberBC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2919023
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-11265Download protocol
Standard Protocols
Click here to view our Standard Protocols