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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD300A Monoclonal antibody

CD300A Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F9C5

Cat no : 67242-1-Ig

Synonyms

CD300A, CD300a molecule, CLM 8, CMRF 35 H9, CMRF 35H, CMRF35 H, CMRF35 H9, CMRF35 like molecule 8, CMRF35H, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, NK inhibitory receptor

Validation Data Gallery

Applications:
  • WB analysis of THP-1 using 67242-1-Ig

    WB analysis of THP-1 using 67242-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67242-1-Ig

    WB analysis of K-562 using 67242-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 67242-1-Ig

    WB analysis of HL-60 using 67242-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human spleen using 67242-1-Ig

    WB analysis of human spleen using 67242-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 67242-1-Ig

    WB analysis of Raji using 67242-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67242-1-Ig

    WB analysis of Jurkat using 67242-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of THP-1 using 67242-1-Ig

    FC experiment of THP-1 using 67242-1-Ig

    1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD300A (67242-1-Ig, Clone:2F9C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, human spleen tissue, Jurkat cells, K-562 cells, Raji cells
Positive FC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67242-1-Ig targets CD300A in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD300A fusion protein Ag5776 相同性解析による交差性が予測される生物種
Full Name CD300a molecule
Calculated molecular weight 299 aa, 33 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC032352
Gene symbol CD300A
Gene ID (NCBI) 11314
RRIDAB_2882521
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CD300A antibody 67242-1-IgDownload protocol
FC protocol for CD300A antibody 67242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols