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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

17A2

Cat no : CL488-65077

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

Validation Data Gallery

Applications:
  • IF Staining of mouse splenocytes using CL488-65077

    IF Staining of mouse splenocytes using CL488-65077

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3 (CL488-65077, Clone: 17A2) at dilution of 1:500.

  • FC experiment of mouse splenocytes using CL488-65077

    FC experiment of mouse splenocytes using CL488-65077

    1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and CoraLite® Plus 488-Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) (right). Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65077-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65077 targets CD3 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen γδTCR-positive T-T hybridoma D1 相同性解析による交差性が予測される生物種
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
RRIDAB_2883163
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
FC protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols