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CoraLite® Plus 488 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65077
Clone No.17A2

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC

AI504783, CD3, Cd3e, CD3epsilon, T3e

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

CL488-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen γδTCR-positive T-T hybridoma D1 相同性解析による交差性が予測される生物種
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
RRIDAB_2883163
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
FC protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Int J Biol Macromol

Polysaccharide-mediated combination therapy enhances anti-tumor effects by promoting the immune cycle of immunogenic cell death

Authors - Xiang-Long Yu
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