CD19 Polyclonal antibody
CD19 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 27914-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
Positive IP detected in | Raji cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Sample-dependent, check data in validation data gallery |
Product Information
27914-1-AP targets CD19 in WB, IP, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD19 fusion protein Ag27404 相同性解析による交差性が予測される生物種 |
Full Name | CD19 molecule |
Calculated molecular weight | 556 aa, 61 kDa |
Observed molecular weight | 95-100 kDa |
GenBank accession number | BC006338 |
Gene symbol | CD19 |
Gene ID (NCBI) | 930 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
Protocols
Product Specific Protocols | |
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WB protocol for CD19 antibody 27914-1-AP | Download protocol |
IP protocol for CD19 antibody 27914-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |