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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD163 Polyclonal antibody

CD163 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16646

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-16646

    IF Staining of human tonsillitis using CL594-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-16646

    IF Staining of human tonsillitis using CL594-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

  • IF Staining of human liver using CL594-16646

    IF Staining of human liver using CL594-16646

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

  • IF Staining of human liver using CL594-16646

    IF Staining of human liver using CL594-16646

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue, human liver tissue
For other applications, we recommend the unconjugated version of this antibody, 16646-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-16646 targets CD163 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD163 fusion protein Ag10029 相同性解析による交差性が予測される生物種
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
RRIDAB_2919836
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Protocols

Product Specific Protocols
IF protocol for CL594 CD163 antibody CL594-16646Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Immunol

Identification of CD101 in Glioma: A Novel Prognostic Indicator Expressed on M2 Macrophages.

Authors - Yuyang Liu
View Article