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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G8

Cat no : CL647-65090

Synonyms

CD16, CD16A, CD16a antigen, CD16B, CD16b antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL647-65090

    FC experiment of human PBMCs using CL647-65090

    1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD16 (CL647-65090, Clone: 3G8) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65090

    FC experiment of human PBMCs using CL647-65090

    1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD16 (CL647-65090, Clone: 3G8) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3-negative lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65090-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65090 targets CD16 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC017865
Gene symbol FCGR3A
Gene ID (NCBI) 2214
RRIDAB_2883675
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD16 antibody CL647-65090Download protocol
Standard Protocols
Click here to view our Standard Protocols