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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Mouse CD134 (OX-86)

CD134 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX-86

Cat no : PE-65136

Synonyms

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

Validation Data Gallery

Applications:
  • FC experiment of BALB/C mouse splenocytes using PE-65136

    FC experiment of BALB/C mouse splenocytes using PE-65136

    1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.2 ug PE Anti-Mouse CD134 (PE-65136, Clone:OX-86). Cells were not fixed.

Tested Applications

Positive FC detected inAnti-CD3/CD28 treated BALB/C mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65136 targets CD134 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
GenBank accession numberBC065782
Gene symbol Tnfrsf4
Gene ID (NCBI) 22163
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Protocols

Product Specific Protocols
FC protocol for PE CD134 antibody PE-65136Download protocol
Standard Protocols
Click here to view our Standard Protocols