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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD133 Monoclonal antibody

CD133 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B8A2

Cat no : CL488-66666

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Validation Data Gallery

Applications:
  • FC experiment of HT-29 using CL488-66666

    FC experiment of HT-29 using CL488-66666

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD133 (CL488-66666, Clone:2B8A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHT-29 cells
For other applications, we recommend the unconjugated version of this antibody, 66666-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66666 targets CD133 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD133 fusion protein Ag13327 相同性解析による交差性が予測される生物種
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa, 80-90 kDa
GenBank accession numberBC012089
Gene symbol PROM1
Gene ID (NCBI) 8842
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD133 antibody CL488-66666Download protocol
Standard Protocols
Click here to view our Standard Protocols