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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD11a/Integrin Alpha L Monoclonal antibody

CD11a/Integrin Alpha L Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E3H12

Cat no : CL594-66256

Synonyms

CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66256-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66256 targets CD11a/Integrin Alpha L in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD11a/Integrin Alpha L fusion protein Ag7813 相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
GenBank accession numberBC008777
Gene symbol ITGAL
Gene ID (NCBI) 3683
RRIDAB_2919972
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
IF protocol for CL594 CD11a/Integrin Alpha L antibody CL594-66256Download protocol
Standard Protocols
Click here to view our Standard Protocols