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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Mouse CD11a (M17/4)

CD11a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

M17/4

Cat no : FITC-65177

Synonyms

Cd11a, integrin alpha L, Itgal, LFA 1, Ly 15, Ly 21

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using FITC-65177

    FC experiment of mouse splenocytes using FITC-65177

    1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Plus Plus Anti-Mouse CD11a (FITC-65177, Clone: M17/4) (red) or 0.5 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen C57BL/6 mouse splenic secondary cytotoxic T cells 相同性解析による交差性が予測される生物種
Full Name integrin alpha L
GenBank accession numberBC145802
Gene symbol Itgal
Gene ID (NCBI) 16408
RRIDAB_2883815
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification Method The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD11a antibody FITC-65177Download protocol
Standard Protocols
Click here to view our Standard Protocols