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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT6B Monoclonal antibody

CCT6B Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11A9

Cat no : 67961-1-Ig

Synonyms

CCT zeta 2, CCT6B, CCTZ 2, Cctz2, Homo sapiens (Human), TCP 1 zeta 2, TSA303

Validation Data Gallery

Applications:
  • WB analysis using 67961-1-Ig

    WB analysis using 67961-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67961-1-Ig

    WB analysis of NIH/3T3 using 67961-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67961-1-Ig

    WB analysis of HSC-T6 using 67961-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67961-1-Ig

    WB analysis of HEK-293 using 67961-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67961-1-Ig

    IHC staining of mouse testis using 67961-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 67961-1-Ig

    IHC staining of mouse testis using 67961-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 67961-1-Ig

    IF Staining of U2OS using 67961-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT6B antibody (67961-1-Ig, Clone: 2F11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of U2OS using 67961-1-Ig

    FC experiment of U2OS using 67961-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human CCT6B (67961-1-Ig, Clone:2F11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67961-1-Ig targets CCT6B in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT6B fusion protein Ag31295 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 6B (zeta 2)
Calculated molecular weight 530 aa, 58 kDa
Observed molecular weight53-58 kDa
GenBank accession numberBC027591
Gene symbol CCT6B
Gene ID (NCBI) 10693
RRIDAB_2918712
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT6B antibody 67961-1-IgDownload protocol
IHC protocol for CCT6B antibody 67961-1-IgDownload protocol
IF protocol for CCT6B antibody 67961-1-IgDownload protocol
FC protocol for CCT6B antibody 67961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols