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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

5F5A6

Cat no : 60264-1-Ig

Synonyms

CCT gamma, CCT3, CCTG, hTRiC5, PIG48, TCP 1 gamma, TRIC5

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 60264-1-Ig

    WB analysis of COLO 320 using 60264-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60264-1-Ig (CCT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 60264-1-Ig

    IHC staining of human liver cancer using 60264-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 60264-1-Ig

    IHC staining of human liver cancer using 60264-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 60264-1-Ig

    IF Staining of HepG2 using 60264-1-Ig

    Immunofluorescent analysis of HepG2 cells using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

  • IF Staining of HepG2 using 60264-1-Ig

    IF Staining of HepG2 using 60264-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (60264-1-Ig, Clone: 5F5A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 60264-1-Ig

    FC experiment of HepG2 using 60264-1-Ig

    1X10^6 HepG2 cells were stained with 0.2ug CCT3 antibody (60264-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

60264-1-Ig targets CCT3 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CCT3 fusion protein Ag0843 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Calculated molecular weight 60 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC006501
Gene symbol CCT3
Gene ID (NCBI) 7203
RRIDAB_2881385
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCT3 antibody 60264-1-IgDownload protocol
IHC protocol for CCT3 antibody 60264-1-IgDownload protocol
IF protocol for CCT3 antibody 60264-1-IgDownload protocol
FC protocol for CCT3 antibody 60264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Front Cell Dev Biol

Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins

Authors - Joshua Linnert
View Article