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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBFB Monoclonal antibody

CBFB Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7F2

Cat no : 67885-1-Ig

Synonyms

CBF beta, CBFB, PEA2 beta, PEBP2 beta, PEBP2B

Validation Data Gallery

Applications:
  • WB analysis using 67885-1-Ig

    WB analysis using 67885-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 67885-1-Ig

    WB analysis of HEK-293 using 67885-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 67885-1-Ig

    FC experiment of K-562 using 67885-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of K-562 using 67885-1-Ig

    FC experiment of K-562 using 67885-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67885-1-Ig targets CBFB in WB, FC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CBFB fusion protein Ag31183 相同性解析による交差性が予測される生物種
Full Name core-binding factor, beta subunit
Calculated molecular weight 182 aa, 22 kDa
GenBank accession numberBC018509
Gene symbol CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

Protocols

Product Specific Protocols
WB protocol for CBFB antibody 67885-1-IgDownload protocol
FC protocol for CBFB antibody 67885-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols