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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated C3/C3b/C3c Polyclonal antibody

C3/C3b/C3c Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21337

Synonyms

ARMD9, ASP, C3, Complement C3, complement component 3, CPAMD1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-21337

    IF Staining of HeLa using CL488-21337

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 C3/C3b/C3c antibody (CL488-21337) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21337-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-21337 targets C3/C3b/C3c in IF applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3/C3b/C3c fusion protein Ag15537 相同性解析による交差性が予測される生物種
Full Name complement component 3
Calculated molecular weight 1663 aa, 187 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC150179
Gene symbol C3
Gene ID (NCBI) 718
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg, and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 C3/C3b/C3c antibody CL488-21337Download protocol
Standard Protocols
Click here to view our Standard Protocols