ATX3,ATXN3 Antibody 67057-1-Ig

適用アプリケーション

アプリケーション:

WB, ELISA
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ATX3,ATXN3 Antibody WB MCF-7 cells 67057-1-Ig

MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

種特異性:

Human, mouse

WB ポジティブ:

MCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue

推奨希釈倍率:

WB : 1:5000-1:50000

Protocols

Product-specific protocols

WB protocol for ATX3,ATXN3 antibody 67057-1-Ig

Standard Protocols

製品情報

由来:

Mouse

精製方法:

Protein A purification

アイソタイプ:

IgG2a

バッファー/保存方法:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

ターゲット情報

免疫原:

ATX3,ATXN3 fusion protein Ag6627

正式名:

ataxin 3

計算分子量:

370 aa, 43 kDa

検出分子量:

41-45 kDa

GenBank accession number:

BC033711

Gene ID (NCBI):

4287

遺伝子シンボル:

ATXN3

別名:

AT3, ataxin 3, ATX3, ATXN3, JOS, MJD, MJD1, SCA3

背景説明

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

WB Figures

ATX3,ATXN3 Antibody WB HEK-293 cells 67057-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATX3,ATXN3 Antibody WB HeLa cells 67057-1-Ig

HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATX3,ATXN3 Antibody WB Jurkat cells 67057-1-Ig

Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATX3,ATXN3 Antibody WB mouse brain tissue 67057-1-Ig

mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATX3,ATXN3 Antibody WB pig brain tissue 67057-1-Ig

pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATX3,ATXN3 抗体 0 文献

Mouse Monoclonal| カタログ番号: 67057-1-Ig |クローン番号: 1F7E10

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