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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of Jurkat using 67057-1-Ig (same clone as 67057-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of pig brain using 67057-1-Ig (same clone as 67057-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of mouse brain using 67057-1-Ig (same clone as 67057-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of HeLa using 67057-1-Ig (same clone as 67057-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of HEK-293 using 67057-1-Ig (same clone as 67057-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

ATX3,ATXN3 Monoclonal antibody, PBS Only

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67057-1-PBS
Clone No.1F7E10

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67057-1-PBS targets ATX3,ATXN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6627

Product name: Recombinant human ATX3,ATXN3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-370 aa of BC033711

Sequence: MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK

相同性解析による交差性が予測される生物種
Full Name ataxin 3
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 41-45 kDa
GenBank accession numberBC033711
Gene Symbol ATXN3
Gene ID (NCBI) 4287
RRIDAB_2882368
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP54252
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.