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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ATPB Monoclonal antibody

ATPB Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11B9

Cat no : CL594-66600

Synonyms

ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66600

    IF Staining of HepG2 using CL594-66600

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ATPB antibody (CL594-66600, Clone: 1A11B9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66600

    FC experiment of HeLa using CL594-66600

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ATPB (CL594-66600, Clone:1A11B9) (red), or 0.4 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL594-66600

    FC experiment of HepG2 using CL594-66600

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ATPB (CL594-66600, Clone:1A11B9) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66600-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66600 targets ATPB in IF, FC (Intra) applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATPB fusion protein Ag11177 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight45-57 kDa
GenBank accession numberBC016512
Gene symbol ATP5B
Gene ID (NCBI) 506
RRIDAB_2920015
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Protocols

Product Specific Protocols
IF protocol for CL594 ATPB antibody CL594-66600Download protocol
Standard Protocols
Click here to view our Standard Protocols