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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ATPB Polyclonal antibody

ATPB Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17247

Synonyms

ATP5B, ATPB, ATPMB, ATPSB

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-17247

    IF Staining of HeLa using CL488-17247

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ATPB antibody (CL488-17247) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17247-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17247 targets ATPB in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPB fusion protein Ag11177 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight45-57 kDa
GenBank accession numberBC016512
Gene symbol ATP5B
Gene ID (NCBI) 506
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ATPB antibody CL488-17247Download protocol
Standard Protocols
Click here to view our Standard Protocols